Acumen has revolutionized PCR so it can be performed directly on the farm
The combination of our compact design, easy-to-use tablet, algorithm and app that walks the user through the process step by step. Now, anyone can run PCR to easily detect pathogens that cause mastitis. We also take the guesswork out of interpreting results - the app will tell you if a pathogen is detected or not. The real power of PCR allows you to TEST BEFORE YOU TREAT.
How does PCR differ from culture?
With PCR there is no subjectivity or guessing as there is in culturing. Often it is difficult to determine which colony on the culture plate is the pathogen that is causing the mastitis infection. which colony is the culprint on the culture plate below? Interpreting culture requires years of expertise and training. It’s not obvious what colony on this plate is causing the infection.
Current literature indicates that up to 30% of bacteria cannot be cultured, which can lead to a false negative, indicating that the cow is infection free when, in fact, she is not. “In no more than 30% of milk samples, from clinical and subclinical bovine mastitis, bacteria fail to grow even after 48 h of conventional culture. The ‘no-growth’ samples are problematic for mastitis laboratories, veterinarians, and dairy producers...We can conclude that all common mastitis bacteria can occur in large quantities in clinical mastitis samples that exhibit no growth in conventional culture, and that the real-time PCR assay is a useful tool for bacteriological diagnosis of such milk samples. ”(Taponen. S, et al. Real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. J Dairy Sci. 2009 Jun; 92(6): 2610-7.)
The New Mastitis Detection Paradigm
Overall Performance Statistics
Table 1 and Table 2 give overall specificity (compared to culture/MALDTI-TOF) and sensitivity values for the Acu-POLARIS pathogen detection. Sensitivity values are determined by spiking clean quarter milk with known concertrations of target organisms.